Instruments

Cell Analyzers

  • Duke West Campus, MSRBII Room 4120
  • Biosafety: BSL2 and BSL2-enhanced 
  • Five lasers: UV (349nm), Violet (405 nm), Blue (488 nm), Yellow-Green (561 nm), Red (637 nm), 
  • 86 Parameters - Blue laser: Forward scatter (FSC), Side scatter (SSC), Axial light loss (ALL) 

Violet laser: Axial light loss (ALL), Side scatter (SSC) 

Fluorescence detectors: 

Spectral arrays – 78 APD detectors paired with algorithmically optimized filter bandwidths covering the full spectrum: UV 349 nm laser – 22 UV detectors, covering 365 nm–860 nm Violet 405 nm laser – 20 Violet detectors, covering 410 nm–860 nm Blue 488 nm laser – 16 Blue detectors, covering 495 nm–860 nm Y/G 561 nm laser – 12 Yellow-green detectors, covering 570 nm–860 nm Red 637 nm laser – 8 Red detectors, covering 645 nm–860 nm

Imaging fluorescence detectors:

Blue laser scatter detectors FL1: 534/46 FL2: 600/60 FL3: 788/225 Forward scatter (FSC) Side scatter (SSC) Axial light loss (ALL)

  • High throughput system: 40 facs tube rack and 96 well plate acquisition system in addition to single facs tubes.
  • A8 configuration 

  • RTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • Five lasers: Blue (488 nm), Green (561 nm), Red (637 nm), Violet (404 nm), and Ultraviolet (355 nm)

  • 31 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 7 fluorescent channels off the Ultraviolet

  • High Throughput System: 96 and 384 well plate-based acquisition in addition to tubes

  • P01 configuration

  • RTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • This analyzer allows users to capture and barcode hundreds to thousands of single cells for genomic and proteomic analysis.

  • Duke West Campus, MSRBII Room 4120
  • Biosafety: BSL2
  • Four lasers: Blue (488 nm), Green (532 nm), Red (642 nm), and Violet (405 nm)
  • 20 parameters: Forward Scatter, Side Scatter, 2 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, and 8 fluorescent channels off the Violet
  • F01 configuration

  • Duke West Campus, MSRBII Room 4120
  • Biosafety: BSL2 and BSL2-Human specimen
  • Four lasers: Blue (488 nm), Green (532 nm), Red (642 nm), and Violet (405 nm)
  • 20 parameters: Foward Scatter, Side Scatter, 2 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 2 fluorescent channels off the Red, and 8 fluorescent channels off the Violet
  • High Throughput System: 96 and 384 well plate-based acquisition in addition to tubes
  • L01 configuration

  • Duke West Campus, MSRBII Room 4120
  • Biosafety: BSL2 and BSL2-Human specimen
  • Three lasers: Blue (488 nm), Red (642 nm), and Violet (405 nm)
  • 15 parameters: Forward Scatter, Side Scatter, 5 fluorescent channels off the Blue, 2 fluorescent channels off the Red, and 6 fluorescent channels off the Violet, that shares many of the same filters as our BD instruments, so will be readily adaptable to many panels designed for our other cytometers
  • PlateCrane EX High Throughput Robotic System: 96, 384 and 1536 well plate-based acquisition
  • Q01 configuration

  • RTP Campus, Room 2104, and Duke West Campus,MSRBII, Room 4116

  • Biosafety: BSL2 and BSL2-Human specimen

Cell Sorters

 

  • RTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • Five lasers: Blue (488 nm), Green (561 nm), Red (638 nm), Violet (402 nm), and Ultraviolet (355 nm)

  • 32 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 8 fluorescent channels off the Ultraviolet

  • 6-way sorting, Index sorting

  • S01 configuration

  • Duke West Campus, GHRB, Room 1071

  • Biosafety: BSL2, BSL2-Human specimen, and BSL3 (please contact RBL facility for more information)

  • Five lasers: Blue (488 nm), Green (561 nm), Red (638 nm), Violet (402 nm), and Ultraviolet (355 nm)

  • 32 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 8 fluorescent channels off the Ultraviolet

  • 6-way sorting, Index sorting

  • S02 configuration

  • Duke West Campus, MSRBII, Room 4038

  • Biosafety: BSL2 and BSL2-Human specimen

  • Four lasers: Blue (488 nm), Green (532 nm), Red (639 nm) and Violet (405 nm)

  • 19 parameters: Forward Scatter, Side Scatter, 2 fluorescent detectors off the Blue, 5 fluorescent detectors off the Green, 3 fluorescent detectors off the Red and 7 fluorescent detectors off the Violet

  • 4-way sorting, Index sorting

  • A02 configuration