Instruments

Cell Analyzers

  • Symphony A5RTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • Five lasers: Blue (488 nm), Green (561 nm), Red (637 nm), Violet (404 nm), and Ultraviolet (355 nm)

  • 31 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 7 fluorescent channels off the Ultraviolet

  • High Throughput System: 96 and 384 well plate-based acquisition in addition to tubes

  • P01 configuration

  • LSRFortessaDuke West Campus, RP1, Room 105

  • Biosafety: BSL2

  • Four lasers: Blue (488 nm), Green (532 nm), Red (642 nm), and Violet (405 nm)

  • 20 parameters: Forward Scatter, Side Scatter, 2 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, and 8 fluorescent channels off the Violet

  • F01 configuration

  • LSRIIDuke West Campus, RP1, Room 105

  • Biosafety: BSL2 and BSL2-Human specimen

  • Four lasers: Blue (488 nm), Green (532 nm), Red (642 nm), and Violet (405 nm)

  • 20 parameters: Foward Scatter, Side Scatter, 2 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 2 fluorescent channels off the Red, and 8 fluorescent channels off the Violet

  • High Throughput System: 96 and 384 well plate-based acquisition in addition to tubes

  • L01 configuration

  • MuseRTP Campus, Room 2104, and Duke West Campus,MSRBII, Room 4116

  • Biosafety: BSL2 and BSL2-Human specimen

  • iQueDuke West Campus, MSRBII, Room 4036

  • Biosafety: BSL2 and BSL2-Human specimen

  • Three lasers: Blue (488 nm), Red (642 nm), and Violet (405 nm)

  • 15 parameters: Forward Scatter, Side Scatter, 5 fluorescent channels off the Blue, 2 fluorescent channels off the Red, and 6 fluorescent channels off the Violet, that shares many of the same filters as our BD instruments, so will be readily adaptable to many panels designed for our other cytometers

  • PlateCrane EX High Throughput Robotic System: 96, 384 and 1536 well plate-based acquisition

  • Q01 configuration

  • RhapsodyRTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • This analyzer allows users to capture and barcode hundreds to thousands of single cells for genomic and proteomic analysis.

Cell Sorters

 

  • Symphony S6RTP Campus, Room 2104

  • Biosafety: BSL2 and BSL2-Human specimen

  • Five lasers: Blue (488 nm), Green (561 nm), Red (638 nm), Violet (402 nm), and Ultraviolet (355 nm)

  • 32 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 8 fluorescent channels off the Ultraviolet

  • 6-way sorting, Index sorting

  • S01 configuration

  • Duke West Symphony S6Campus, GHRB, Room 1071

  • Biosafety: BSL2, BSL2-Human specimen, and BSL3 (please contact RBL facility for more information)

  • Five lasers: Blue (488 nm), Green (561 nm), Red (638 nm), Violet (402 nm), and Ultraviolet (355 nm)

  • 32 parameters: Foward Scatter, Side Scatter, 6 fluorescent channels off the Blue, 5 fluorescent channels off the Green, 3 fluorescent channels off the Red, 8 fluorescent channels off the Violet, and 8 fluorescent channels off the Ultraviolet

  • 6-way sorting, Index sorting

  • S02 configuration

  • Aria IIDuke West Campus, MSRBII, Room 4038

  • Biosafety: BSL2 and BSL2-Human specimen

  • Four lasers: Blue (488 nm), Green (532 nm), Red (639 nm) and Violet (405 nm)

  • 19 parameters: Forward Scatter, Side Scatter, 2 fluorescent detectors off the Blue, 5 fluorescent detectors off the Green, 3 fluorescent detectors off the Red and 7 fluorescent detectors off the Violet

  • 4-way sorting, Index sorting

  • A02 configuration