Protein Production Facility Core

Quality-controlled protein production for Research Use Only:  

DHVI PPF transiently transfects 293 or CHO mammalian cells with plasmids for recombinant expression.  All proteins are run on SDS-Page and Western Blot for quality control analysis, and proteins that are not at least 85-90% pure are further purified. 

• Nickel, lectin, protein A, and Kappa select purification methods are available.

• Recombinant Viral Envelope Proteins (1 or 4 Liter Run Scale Production)

• Recombinant Immunoglobulin (Ab or Fab; 1 or 4 Liter Run Scale Production)

Protein purification and Quality Testing

• FPLC-SEC Purification
• Sterility, endotoxin and mycoplasma testing available

Use Fees/Rates

Current approved rates for Duke Investigators can be seen in CoreResearch@Duke (Core D-0078)

CoreResearch@Duke can also be used to request an ESTIMATE for grant or contract proposals

Service Requests and Estimates

Internal Duke users:  

• How to submit a New Request (instructions)

External Non-Profit Institution users:

• A signed Research Analysis Quote and Agreement (RAQA) and Purchase Order (PO) will be needed from your institution
• Contact PPF Director for a price quote to get the process started.

Non-Duke For-Profit Entity users:

• Duke Shared Resources can only provide services to For-Profit Entities under a signed Sponsored Research Agreement (SRA)
• The collaborative research project must have a defined research question, scope of work, budget, and timeline.
• Contact PPF Director to get the process started

DHVI - Protein Production Facility Data Management Plan