Technical Help


  • Fluorofinder is a free flow cytometry resource to streamline design of panels on any DHVI cytometer.
  • Antibody Search Tool: Real time feedback on over 145,000 reagents and 354 fluorochromes available on the cloud-based tool
  • Customizable: Multicolor panels that work with DHVI cytometer configurations
  • Free: Individual accounts at no cost to academic/nonprofit institutions & researchers
  • For a quick walk through the panel building process, watch this video.

BD Horizon Guided Panel Solution (GPS)

  • GPS provides a guided workflow for reagent selection based on the principles of panel design.
  • Use is free but users must create an account. Panels can be saved. 

Spectrum Viewer

  • Helpful tool from BD to determine optimal laser and filter combinations for different fluorochromes



  • FlowJo is the leading analysis platform for single-cell flow and mass cytometry analysis
  • Individual licenses can be obtained through the DHVI flow cytometry facility. Request a license here.


  • Cytobank is a cloud-based platform specializing in the analysis and visualization of multiple single-cell data sets simultaneously.
  • Tools include SPADE, viSNE, and CITRUS analyses.
  • Monthly fee; free 30-day trial.


  • SPICE is a data mining software application that analyzes large FlowJo® data sets from polychromatic flow cytometry and organizes the normalized data graphically.


Open Flow: Intro to Cell Sorting

Open Flow: Flourescence Compensation in Diva

  • Reviews the principles behind compensation and how it is carried out on a BD Fortessa analyzer, using a live-run with a four color experiment. 
  • A recording of this session can be found on their YouTube

Open Flow: Multicolor Troubleshooting in Diva

  • Focuses on rules of compensation and how our data can suffer when we do not follow them.
  • A recording of this fourth session can be found on their YouTube