Flow Cytometry at EQAPOL

The EQAPOL Flow Cytometry program assesses the proficiency of NIAID/DAIDS-supported and other interested research laboratories at performing multiple flow cytometry protocols.  Proficiency is assessed with the goal of helping all participating laboratories to perform the assay. Participating laboratories are required to complete two External Proficiency (EP) rounds per year

For each EP round, EQAPOL provides all of the reagents to run each assay including: PBMCs selected based on their cytokine production following stimulation, stimulation plates containing lyophilized peptides and staining plates containing lyophilized antibodies and all other necessary instrument set-up and staining reagents. Sites are required to run the assay using the EQAPOL-provided kit and protocol. Currently, the EQA program assess proficiency using an 8-color Intracellular Cytokine Staining (ICS) assay that measures IFN-γ, IL-2, TNF-α and CD107α.

Sites are required to run the EQAPOL-provided flow cytometry assay and report both site-analyzed data and raw FCS files. Statistical assessment, troubleshooting, final report and discussion of results occur between participating laboratories and EQAPOL/NIAID. For each EP, sites are provided with a site-specific report. Participating laboratories are blinded with a unique EQAPOL site ID, and each laboratory is unblinded with respect to only their data sets.

The EQAPOL Flow Cytometry Proficiency Testing Program was recently accredited to the International Organization for Standardization/ International Electrotechnical Commission (ISO/IEC) 17043:2010 standards by the American Association for Laboratory Accreditation (A2LA).

Accredited Symbol

A2LA Cert 3614.01 

Upcoming EP Testing


  • September 2019: EP12 shipments begin

Completed EP Testing


  • August 7, 2017: EP11 shipments begin
  • October 3, 2017: EP11 data collection closes
  • December 14, 2017: EP11 reports released


  • January 17, 2017: EP10 shipments begin
  • March 10,2017: EP10 data collection closes
  • June 29, 2017: EP10 reports released


  • May 23, 2016: EP9 shipments begin
  • June 27, 2016: EP9 data collection closes
  • September 27, 2016: EP9 reports released


  • November 9, 2015: EP8 shipments begin
  • January 8, 2016: EP8 data collection closes
  • March 17, 2016: EP8 reports released


  • February 16, 2015: EP7 shipments begin
  • April 3, 2015: EP7 data collection closes
  • August 24, 2015: EP7 reports released


  • August 11, 2014: EP6 shipments begin
  • October 23, 2014: EP6 data collection close
  • December 10, 2014: EP6 reports released


  • January 27, 2014: EP5 shipments begin
  • March 15, 2014: EP5 data collection closes
  • May 26, 2014: E5 reports released


  • July 29, 2013: EP4 shipments begin
  • September 10, 2013: EP4 data collection closes
  • November 18, 2013: EP reports released


  • November 27, 2012: EP3 shipments begin
  • March 12, 2013: EP3 data collection closes
  • June 11, 2013: EP3 reports released


  • April 02, 2012: EP2 shipments begin
  • July 20, 2012: EP2 data collection closes
  • September 06, 2012: EP2 reports released


  • July 11, 2011: EP1 shipments begin
  • March 05, 2012: EP1 reports released

United States

Nonhuman Primate Core Cellular Immunology Laboratory (NHP-CIL)
18703 Three Rivers Road
Covington, Louisiana 70433
United States
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Baylor Institute for Immunology Research
3410 Worth Street
Suite 600
Dallas, Texas 75246
United States
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UNC - Department of Microbiology and Immunology
120 Mason Farm Road
RM 2023A, Genetic Medicine Building, CB#7042
Chapel Hill, North Carolina 27599
United States
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Duke Brain Tumor Immunotherapy Program
213 Alex H. Sands Jr. Research Building
Room 204
Durham, North Carolina 27710
United States
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ABL GCLP Laboratory
9800 Medical Center Drive
Building D
Rockville, Maryland 20850
United States
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Rouphael Emory VTEU Laboratory
500 Irvin Court
Suite 100
Decatur, Georgia 30030
United States
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Southwest National Primate Research Center (SNPRC) - Texas Biomed
7620 NW Loop 410
San Antonio , Texas 78227
United States
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Cellular Immunology Lab (CIL) - US Military HIV Research Program (USMHRP) Immunology Laboratory
503 Robert Grant Avenue
Silver Spring, Maryland 20910
United States
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UMASS Medical Center Clinical Laboratory
373 Plantation Street - Suite 318, Biotech II
Worcester, Maryland 01605
United States
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NIAID Vaccine Immune T-Cell and Antibody Laboratory (NVITAL)
NIAID Vaccine Immune T-Cell and Antibody Laboratory (NVITAL)
Suite 150
Gaithersburg, Maryland 20878
United States
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Magee-Womens Research Institute
204 Craft Ave
Pittsburgh, Pennsylvania 15213
United States
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HIV Vaccine Trial Network (HVTN)
1100 Fairview Avenue North, LE-200
Seattle, Washington 98109
United States
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Custom Tech Team @ BD
11077 North Torrey Pines Road
La Jolla, California 92037
United States
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13 Taft Court
Rockville, Maryland 20850
United States
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South Africa

South African Tuberculosis Vaccine Research Initiative (SATVI)
Anzio Road, Observatory
Wernher Beit South Building, University of Cape Town Faculty of Health Sciences
Cape Town 7925
South Africa
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Cape Town HVTN Immunology Laboratory
Wembley Square 3
McKenzie Street Gardens
Cape Town, Western Cape 8001
South Africa
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SUN Immunology Research Laboratory
Nelita Du Plessis-Burger
Tygerberg Campus Cape Town
Cape Town, Western Cape 7550
South Africa
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Sanofi Pasteur - Immuno-Microbiology Platform
Bat. X North, 212 1541 Avenue Marcel Mérieux
Marcy l'Etoile 69280
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Caprion Biosciences Inc.
201 ave President-Kennedy
Suite PK-3900
Montreal, Quebec H2X 3Y7
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National Immune Monitoring Laboratory (NIML)
2901 rue Rachel Est Suite # 22
Montreal H1W 4A4
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US Military HIV Research Program (USMHRP)
Hospital Road - P.O. Box 2410
Mbeya Tanzania
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Uganda Virus Research Institute
Plot 51-59 Nakiwogo Road
P.O. Box 49
Entebbe Uganda
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Makerere University Walter Reed Project (MUWRP)
P.O. Box 16524
Old Mulago Hill Road
, Kampala Uganda
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United Kingdom

Imperial College London
369 Fulham Road
London SW109NH
United Kingdom
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Imperial College of London
London SW7 2AZ
United Kingdom
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Department of Retrovirology, AFRIMS
315/6 Rajvithi Road
Bankok 10400
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Site reports for EQAPOL Flow Cytometry can be accessed using the EQAPOL web-based application (https://eqapolapp.dhvi.duke.edu).