The EQAPOL ELISpot EQA program assesses the proficiency of NIAID/DAIDS-supported and other interested research laboratories at performing an interferon-γ (IFN-γ) ELISpot assay. Proficiency is assessed with the goal of helping all participating laboratories to perform the assay. Participating laboratories are required to complete two External Proficiency (EP) rounds per year.

As part of the program, EQAPOL provides the reagents for sites to run their own in-house, IFN-γ ELISpot assay using peripheral blood mononuclear cells (PBMCs) and standardized peptide pools. The PBMCs selected for each EP round have varying reactivities to the provided peptides. In addition to these standardized reagents, the materials to run an EQAPOL IFN-γ ELISpot assay - IFN-γ-coated plates, secondary antibodies, and a detection reagent - are provided in each send-out.

Sites are required to use their in-house assay using the PBMCs and peptides provided by EQAPOL. They are also required to run the EQAPOL-provided IFN-γ ELISpot assay using the provided PBMCs and peptides. Statistical assessment, troubleshooting, final report and discussion of results occur between participating laboratories and EQAPOL/NIAID. For each EP, sites are provided with a site-specific report. Participating laboratories are blinded with a unique EQAPOL site ID, and each laboratory is unblinded with respect to only their data sets.

The EQAPOL ELISpot Proficiency Testing Program was recently accredited to the International Organization for Standardization/ International Electrotechnical Commission (ISO/IEC) 17043:2010 standards by the American Association for Laboratory Accreditation (A2LA).

Accredited Symbol

A2LA Cert 3614.01

Upcoming EP Testing


  • August 2019: EP18 Shipments begin

Completed EP Testing


  • February 2019: EP17 shipments begin
  • April 2019: EP17 data collection closes
  • May 2019: EP17 Reports released


  • August 2018: EP16 shipments begin 
  • October 2018: EP16 data collection closes
  • December 2018: EP16 Reports Released   


  • March 2018: EP15 shipments begin 
  • June 2018: EP15 data collection closes 
  • July 2018: EP15 reports released 


  • October 2, 2017: EP14 shipments begin
  • Nov 17, 2017: EP14 data collection closes
  • Dec 21, 2017: EP14 reports released


  • March 20, 2017: EP13 shipments begin
  • May 2, 2017: EP13 data collection closes
  • June 30, 2017: EP13 reports released


  • September 19, 2016: EP12 shipments begin
  • November 29, 2016: EP12 data collection closes
  • December 21, 2016: EP12 reports released 


  • March 28, 2016: EP11 Shipments Begin
  • May 24, 2016: EP11 data collection closes
  • June 23, 2016: EP11 reports released 


  • September 21, 2015: EP10 Shipments Begin
  • October 27, 2015: EP10 data collection closes
  • December 21, 2015: EP10 reports released


  • May 18, 2015: EP9 Shipments Begin
  • July 6, 2015: EP9 data collection closes
  • August 12, 2015: EP9 reports released


  • November 10, 2014: EP8 shipments begin
  • January 8, 2015: EP8 data collection closes
  • February 26, 2015: EP8 reports released


  • May 19, 2014: EP7 shipments begin
  • June 30, 2014: EP7 data collection closes
  • August 04, 2014: EP7 reports released


  • November 11, 2013: EP6 shipments begin
  • December 19, 2013: EP6 data collection closes
  • January 21, 2014: EP6 reports released


  • June 18, 2013: EP5 shipments begin
  • July 25, 2013: EP5 data collection closes
  • August 23, 2013: EP5 reports released


  • January 21, 2013: EP4 shipments begin
  • March 01, 2013: EP4 data collection closes
  • April 04, 2013: EP4 reports released


  • April 23, 2012: EP3 shipments begin
  • June 04, 2012: EP3 data collection closes
  • July 26, 2012: EP3 reports released  


  • December 5, 2011: EP2 shipments begin
  • January 31, 2012: EP2 data collection closes
  • March 20, 2012: EP2 reports released 


  • June 20, 2011: EP1 shipments begin
  • August 26, 2011: EP1 data collection closes
  • October 6, 2011: EP1 reports released

Site reports for EQAPOL ELISpot can be accessed using the EQAPOL web-based application (https://eqapolapp.dhvi.duke.edu).

United States

Nonhuman Primate Core Cellular Immunology Laboratory (NHP-CIL)
18703 Three Rivers Road
Covington, Louisiana 70433
United States
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Covance CLS
8211 SciCor Drive
Indianapolis, Indiana 46214
United States
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Baylor Institute for Immunology Research
3410 Worth Street
Suite 600
Dallas, Texas 75246
United States
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UNC - Department of Microbiology and Immunology 
120 Mason Farm Road
RM 2023A Genetic Medicine Building, CB#7042
Chapel Hill, North Carolina 27599
United States
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Duke Brain Tumor Immunotherapy Program
213 Alex H. Sands Jr. Research Building
Durham, North Carolina 27710
United States
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Ragon Institute of MGH, MIT and Harvard
400 Technology Square, Room 934
Cambridge, Massachusetts 02139
United States
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NIAID Vaccine Immune T-Cell and Antibody Laboratory (NVITAL)
9 West Watkins Mill Road
Suite 150
Gaithersburg, Maryland 20878
United States
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Endpoints Laboratory Human Vaccine Trials Network (HVTN)
1100 Fairview Avenue North, LE-200
P.O, Box 19024
Seattle, Washington 98109
United States
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Division of Vaccine Research Beth Israel Deaconess Medical Center (BIDMC)
3 Blackfan Circle
Boston, Massachusetts 02115
United States
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Custom Tech Team @ BDS
11077 North Torrey Pines Road
La Jolla, California 92037
United States
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CIL Henry M. Jackson Foundation Department of Vaccine Research and Development US Military HIV Research Program (USMHRP)
13 Taft Court
Rockville, Maryland 20850
United States
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9800 Medical Center Drive, Building D
Rockville, Maryland 20850
United States
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ABL Lyon
321 Avenue Jean Jaures
Lyon 69007
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MRC/UVRI Uganda Research Unit on AIDS
Plot 51-59 Nakiwogo Road
P.O. Box 49
Entebbe, Wakiso Uganda
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Laboratory of AIDS Immunopathogenesis CHUV
Avenue Beaumont, 29 / BT02 - 224
Lausanne 1011
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United Kingdom

369 Fulham Road
London SW10 9NH
United Kingdom
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Fraunhofer IBMT
Industriestr. 5
Sulzbach D-66280
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DoD Laboratory in Tanzania NIMR Mbeya Medical Research Program
Hospital Road - P.O. Box 2410
, Mbeya Tanzania
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DoD Laboratory in Tanzania
PO Box 2410
Mbeya Tanzania
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Department of Retrovirology, AFRIMS American Embassy
120 Wireless Road
Praturnwan, Bangkok Thailand
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Department of Retrovirology
120 Wireless Road
Bangkok Thailand
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