Luminex

The EQAPOL Luminex proficiency program was initiated as a collaboration between NIAID and the Cancer Immunotherapy Consortium (CIC). The EQAPOL Luminex EQA program assesses the proficiency of NIAID/DAIDS-supported and other interested research laboratories at performing an bead-based Luminex assay. Proficiency is assessed with the goal of helping all participating laboratories to perform the assay. Participating laboratories are required to complete two External Proficiency (EP) rounds per year.

For each EP round, Luminex program participants receive a de-identified custom 5-plex Luminex-bead assay kit (blinded to the reagent manufacturer), which includes all necessary reagents to run the assay, from a vendor selected by CIC/NIAID. In addition to these kits, sites will receive 21 test samples containing known amounts of the five cytokines measured by the assay in a human AB serum matrix.

Sites are required to run the EQAPOL-provided Luminex assay and report both site-reported observed concentrations (pg/mL) of each samples, and raw MFI data. Statistical assessment, troubleshooting, final report and discussion of results occur between participating laboratories and EQAPOL/NIAID. For each EP, sites are provided with a site-specific report. Participating laboratories are blinded with a unique EQAPOL site ID, and each laboratory is unblinded with respect to only their data sets.

The EQAPOL Luminex Proficiency Testing Program was recently accredited to theInternational Organization for Standardization/ International Electrotechnical Commission (ISO/IEC) 17043:2010 standards by the American Association for Laboratory Accreditation (A2LA).

Accredited Symbol

A2LA Cert 3614.01 

Upcoming EP Testing

    EP18

    • October 2019: EP18 shipments begin

      Completed EP Testing

      EP17 

      • April 2019: EP17 shipments begin
      • May 2019: EP17 data collection closes
      • June 2019: EP17 reports released

      EP16 

      • October 2018: EP16 shipments begin
      • November 2018: EP16 data collection closes 
      • December 2018: EP16 reports released

      EP15 

      • April 2018: EP15 shipments begin
      • June 2018: EP15 data collection closes 
      • July 2018: EP15 reports released

      EP14

      • October 30, 2017: EP14 shipments begin
      • Dec 15, 2017: EP14 data collection closes
      • Jan 30, 2018: EP14 reports released

      EP13 

      • April 10, 2017: EP13 shipments begin
      • July 6, 2017: EP13 data collection closes
      • July 19, 2017: EP13 reports released

      EP12

      • October 10, 2016: EP12 shipments begin
      • November 9, 2016: data collection closes 
      • December 15, 2016: EP11 reports released 

      EP11

      • April 18, 2016: EP11 shipments begin
      • May 24, 2016: EP11 data collection closes
      • June 21, 2016: EP11 reports released 

      EP10

      • October 12, 2015: EP10 shipments begin
      • November 23, 2015: EP10 data collection closes
      • December 17, 2015: EP10 reports released

      EP9

      • April 20, 2015: EP9 shipments begin
      • May 19, 2015: EP9 data collection closes
      • June 10, 2015: EP9 reports released

      EP8

      • October 13, 2014: EP8 shipments begin
      • November 21, 2014: EP8 data collection closes
      • December 18, 2014: EP8 reports released

      EP7

      • April 21, 2014: EP7 shipments begin
      • May 29,  2014: EP7 data collection closes
      • June 20, 2014: EP7 reports released

      EP6

      • October 14, 2013: EP6 shipments begin
      • November 22,  2013: EP6 data collection closes
      • December 18, 2013: EP6 reports released

      EP5

      • June 03, 2013: EP5 shipments begin
      • July  09,  2013: EP5 data collection closes
      • July 29, 2013: EP5 reports released

      EP4

      • February 11, 2013: EP4 shipments begin
      • March 28, 2013: EP4 data collection closes
      • April 23, 2013: EP4 reports released

      EP3

      • June 11, 2012: EP3 shipments begin
      • July 23, 2013: EP3 data collection closes
      • September 26, 2013: EP3 reports released

      EP2

      • March 19, 2012: EP2 shipments begin
      • May 01, 2012: EP2 data collection closes
      • June 06, 2012: EP2 reports released

      EP1

      • September 6, 2011: EP1 shipments begin
      • January 9, 2012: EP1 reports released

      Luminex Site-specific reports are available on the EQAPOL web-based system (https://eqapolapp.dhvi.duke.edu).

      United States

      Immunoregulatory Specialty Laboratory (IRSL)
      3550 Terrace Street
      808 Scaife Hall
      Pittsburgh, Pennsylvania 15261
      United States
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      Pathogen Detection and Quantification Core (PDQC)
      18703 Three Rivers Road
      Building 5, Room 106
      Covington, Louisiana 70433
      United States
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      USU-WR-SUR Laboratory
      8930 Brown Drive
      Bethesda, Maryland 20889
      United States
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      Substrate Services Core & Research Support (SSCRS) - Duke University
      203 Research Dr. MSRB I
      Durham, North Carolina 27710
      United States
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      Oregon Health & Science University
      3181 SW Sam Jackson Park Road
      Dock 14 (CDRC 2250)
      Portland, Oregon 97239
      United States
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      ABL GCLP Laboratory
      9800 Medical Center Drive
      Rockville, Maryland 20850
      United States
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      University of Pittsburgh
      5117 Centre Avenue, Suite 1.27
      Hillman Cancer Center Research Pavilion
      Pittsburgh, Pennsylvania 15213
      United States
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      University of Miami, Immunologic Monitoring Laboratory
      1600 NW 10th Ave, Room 8155
      Rosenstiel Medical Science Building
      Miami, Florida 33136
      United States
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      University of Hawai'i Center for AIDS
      BSB325, 651 Ilalo St.
      Honolulu, Hawaii 96826
      United States
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      The Rockefeller University
      1230 York Avenue
      New York, New York 10065
      United States
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      Stanford University
      299 Campus Drive
      Fairchild Building, Room D-033
      Stanford, California 94305
      United States
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      SNPRC - Texas Biomed
      7620 NW Loop 410
      San Antonio , Texas 78227
      United States
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      Roswell Park Cancer Institute
      Elm and Carlton Sts, CCC-324
      Buffalo, New York 14263
      United States
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      Ragon Institute- Human Immune Monitoring Core
      400 Technology Square, Room 934
      Cambridge, Massachusetts 02139
      United States
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      Pfizer Vaccine East - Assay Development
      401 North Middletown Road
      B. 180 Room 265
      Pearl River, New York 10965
      United States
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      Memorial Sloan Kettering Cancer Center
      411 East 67th Street
      Schwartz-724
      New York, New York 10065
      United States
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      Magee-Womens Research Institute
      204 Craft Ave, Room A530
      Pittsburgh, Pennsylvania 15213
      United States
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      Johns Hopkins University
      417 North Caroline Street
      Room 302
      Baltimore, Maryland 21231
      United States
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      Dartmouth College
      14 Engineering Drive, Cummings Rm 120
      C/O Mary Kay Brown
      Hanover, New Hampshire 03755
      United States
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      Dartmouth College
      1 Medical Center Drive
      710 Rubin Building
      Lebanon, New Hampshire 03756
      United States
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      Vitalent Research Institute
      270 Masonic Avenue
      San Francisco, California 94418
      United States
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      Baylor Institute for Immunology Research
      3434 Live Oak Street
      Dallas, Texas 75204
      United States
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      AcuImmune
      21818 Craggy View Street
      Suite 203
      Chatsworth, California 91311
      United States
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      Duke University Medical Center
      1312 Campus Drive
      Durham, North Carolina 27705
      United States
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      Australia

      360biolabs
      85 Commercial Road
      Melbourne, Victoria 3004
      Australia
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      CSL Limited
      45 Poplar Road
      Parkville, Victoria 3052
      Australia
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      South Africa

      SUN Immunology Research Laboratory
      Department of Biomedical Sciences, Faculty of Health Sciences
      University of Stellenbosch, Tygerberg Campus
      Cape Town, Western Cape 7505
      South Africa
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      France

      Sanofi Pasteur - Immuno-Microbiology Platform
      Bat. X North, 212 1541 Avenue Marcel Mérieux
      Marcy l'Etoile, Rhone (69) 69280
      France
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      Japan

      Radiations Effects Research Foundation (RERF) Luminex Laboratory
      5-2 Hijiyama Park, Minami Ward
      Hiroshima 732-0815
      Japan
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      Uganda

      MRC/UVRI Uganda Research Unit on AIDS
      Plot 51-59 Nakiwogo Road, P.O.Box 49
      Entebbe, Wakiso Uganda
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      Canada

      McMaster University
      MDCL Room 4074 1200 Main Street West
      Hamilton, Ontario L8N 3Z5
      Canada
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      Germany

      Institute of Medical Immunology - IISL - Charité University Medicine Berlin, Campus Virchow-Klinikum
      Forschungshaus, Forum 4 Westring 3
      Berlin 13353
      Germany
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      Spain

      Centre de Recerca en Salut Internacional de Barcelona (CRESIB) (Barcelona Centre for International Health Research)
      Edifici CEK
      C/ Rosselló nº 153, 1ª Planta
      , Barcelona 08036
      Spain
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      Czech Republic

      Central Laboratory of Immunoanalysis
      ONM-OID, FN Plzen, E.Benese 13
      Plzen 305 99
      Czech Republic
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      United Kingdom

      University of Oxford
      Wellington Square
      Oxford OX1 2JD
      United Kingdom
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