Luminex

The EQAPOL Luminex proficiency program was initiated as a collaboration between NIAID and the Cancer Immunotherapy Consortium (CIC). The EQAPOL Luminex EQA program assesses the proficiency of NIAID/DAIDS-supported and other interested research laboratories at performing an bead-based Luminex assay. Proficiency is assessed with the goal of helping all participating laboratories to perform the assay. Participating laboratories are required to complete two External Proficiency (EP) rounds per year.

For each EP round, Luminex program participants receive a de-identified custom 5-plex Luminex-bead assay kit (blinded to the reagent manufacturer), which includes all necessary reagents to run the assay, from a vendor selected by CIC/NIAID. In addition to these kits, sites will receive 21 test samples containing known amounts of the five cytokines measured by the assay in a human AB serum matrix.

Sites are required to run the EQAPOL-provided Luminex assay and report both site-reported observed concentrations (pg/mL) of each samples, and raw MFI data. Statistical assessment, troubleshooting, final report and discussion of results occur between participating laboratories and EQAPOL/NIAID. For each EP, sites are provided with a site-specific report. Participating laboratories are blinded with a unique EQAPOL site ID, and each laboratory is unblinded with respect to only their data sets.

The EQAPOL Luminex Proficiency Testing Program was recently accredited to theInternational Organization for Standardization/ International Electrotechnical Commission (ISO/IEC) 17043:2010 standards by the American Association for Laboratory Accreditation (A2LA).

Accredited Symbol

A2LA Cert 3614.01 

Upcoming EP Testing

EP18

  • October 2019: EP18 shipments begin

Completed EP Testing

EP17 

  • April 2019: EP17 shipments begin
  • May 2019: EP17 data collection closes
  • June 2019: EP17 reports released

EP16 

  • October 2018: EP16 shipments begin
  • November 2018: EP16 data collection closes 
  • December 2018: EP16 reports released

EP15 

  • April 2018: EP15 shipments begin
  • June 2018: EP15 data collection closes 
  • July 2018: EP15 reports released

EP14

  • October 30, 2017: EP14 shipments begin
  • Dec 15, 2017: EP14 data collection closes
  • Jan 30, 2018: EP14 reports released

EP13 

  • April 10, 2017: EP13 shipments begin
  • July 6, 2017: EP13 data collection closes
  • July 19, 2017: EP13 reports released

EP12

  • October 10, 2016: EP12 shipments begin
  • November 9, 2016: data collection closes 
  • December 15, 2016: EP11 reports released 

EP11

  • April 18, 2016: EP11 shipments begin
  • May 24, 2016: EP11 data collection closes
  • June 21, 2016: EP11 reports released 

EP10

  • October 12, 2015: EP10 shipments begin
  • November 23, 2015: EP10 data collection closes
  • December 17, 2015: EP10 reports released

EP9

  • April 20, 2015: EP9 shipments begin
  • May 19, 2015: EP9 data collection closes
  • June 10, 2015: EP9 reports released

EP8

  • October 13, 2014: EP8 shipments begin
  • November 21, 2014: EP8 data collection closes
  • December 18, 2014: EP8 reports released

EP7

  • April 21, 2014: EP7 shipments begin
  • May 29,  2014: EP7 data collection closes
  • June 20, 2014: EP7 reports released

EP6

  • October 14, 2013: EP6 shipments begin
  • November 22,  2013: EP6 data collection closes
  • December 18, 2013: EP6 reports released

EP5

  • June 03, 2013: EP5 shipments begin
  • July  09,  2013: EP5 data collection closes
  • July 29, 2013: EP5 reports released

EP4

  • February 11, 2013: EP4 shipments begin
  • March 28, 2013: EP4 data collection closes
  • April 23, 2013: EP4 reports released

EP3

  • June 11, 2012: EP3 shipments begin
  • July 23, 2013: EP3 data collection closes
  • September 26, 2013: EP3 reports released

EP2

  • March 19, 2012: EP2 shipments begin
  • May 01, 2012: EP2 data collection closes
  • June 06, 2012: EP2 reports released

EP1

  • September 6, 2011: EP1 shipments begin
  • January 9, 2012: EP1 reports released

Luminex Site-specific reports are available on the EQAPOL web-based system (https://eqapolapp.dhvi.duke.edu).

United States

Immunoregulatory Specialty Laboratory (IRSL)
3550 Terrace Street
808 Scaife Hall
Pittsburgh, Pennsylvania 15261
United States
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Pathogen Detection and Quantification Core (PDQC)
18703 Three Rivers Road
Building 5, Room 106
Covington, Louisiana 70433
United States
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USU-WR-SUR Laboratory
8930 Brown Drive
Bethesda, Maryland 20889
United States
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Substrate Services Core & Research Support (SSCRS) - Duke University
203 Research Dr. MSRB I
Durham, North Carolina 27710
United States
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Oregon Health & Science University
3181 SW Sam Jackson Park Road
Dock 14 (CDRC 2250)
Portland, Oregon 97239
United States
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ABL GCLP Laboratory
9800 Medical Center Drive
Rockville, Maryland 20850
United States
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University of Pittsburgh
5117 Centre Avenue, Suite 1.27
Hillman Cancer Center Research Pavilion
Pittsburgh, Pennsylvania 15213
United States
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University of Miami, Immunologic Monitoring Laboratory
1600 NW 10th Ave, Room 8155
Rosenstiel Medical Science Building
Miami, Florida 33136
United States
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University of Hawai'i Center for AIDS
BSB325, 651 Ilalo St.
Honolulu, Hawaii 96826
United States
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The Rockefeller University
1230 York Avenue
New York, New York 10065
United States
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Stanford University
299 Campus Drive
Fairchild Building, Room D-033
Stanford, California 94305
United States
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SNPRC - Texas Biomed
7620 NW Loop 410
San Antonio , Texas 78227
United States
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Roswell Park Cancer Institute
Elm and Carlton Sts, CCC-324
Buffalo, New York 14263
United States
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Ragon Institute- Human Immune Monitoring Core
400 Technology Square, Room 934
Cambridge, Massachusetts 02139
United States
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Pfizer Vaccine East - Assay Development
401 North Middletown Road
B. 180 Room 265
Pearl River, New York 10965
United States
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Memorial Sloan Kettering Cancer Center
411 East 67th Street
Schwartz-724
New York, New York 10065
United States
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Magee-Womens Research Institute
204 Craft Ave, Room A530
Pittsburgh, Pennsylvania 15213
United States
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Johns Hopkins University
417 North Caroline Street
Room 302
Baltimore, Maryland 21231
United States
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Dartmouth College
14 Engineering Drive, Cummings Rm 120
C/O Mary Kay Brown
Hanover, New Hampshire 03755
United States
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Dartmouth College
1 Medical Center Drive
710 Rubin Building
Lebanon, New Hampshire 03756
United States
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Vitalent Research Institute
270 Masonic Avenue
San Francisco, California 94418
United States
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Baylor Institute for Immunology Research
3434 Live Oak Street
Dallas, Texas 75204
United States
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AcuImmune
21818 Craggy View Street
Suite 203
Chatsworth, California 91311
United States
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Duke University Medical Center
1312 Campus Drive
Durham, North Carolina 27705
United States
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Australia

360biolabs
85 Commercial Road
Melbourne, Victoria 3004
Australia
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CSL Limited
45 Poplar Road
Parkville, Victoria 3052
Australia
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South Africa

SUN Immunology Research Laboratory
Department of Biomedical Sciences, Faculty of Health Sciences
University of Stellenbosch, Tygerberg Campus
Cape Town, Western Cape 7505
South Africa
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France

Sanofi Pasteur - Immuno-Microbiology Platform
Bat. X North, 212 1541 Avenue Marcel Mérieux
Marcy l'Etoile, Rhone (69) 69280
France
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Japan

Radiations Effects Research Foundation (RERF) Luminex Laboratory
5-2 Hijiyama Park, Minami Ward
Hiroshima 732-0815
Japan
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Uganda

MRC/UVRI Uganda Research Unit on AIDS
Plot 51-59 Nakiwogo Road, P.O.Box 49
Entebbe, Wakiso Uganda
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Canada

McMaster University
MDCL Room 4074 1200 Main Street West
Hamilton, Ontario L8N 3Z5
Canada
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Germany

Institute of Medical Immunology - IISL - Charité University Medicine Berlin, Campus Virchow-Klinikum
Forschungshaus, Forum 4 Westring 3
Berlin 13353
Germany
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Spain

Centre de Recerca en Salut Internacional de Barcelona (CRESIB) (Barcelona Centre for International Health Research)
Edifici CEK
C/ Rosselló nº 153, 1ª Planta
, Barcelona 08036
Spain
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Czech Republic

Central Laboratory of Immunoanalysis
ONM-OID, FN Plzen, E.Benese 13
Plzen 305 99
Czech Republic
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United Kingdom

University of Oxford
Wellington Square
Oxford OX1 2JD
United Kingdom
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