Flow Cytometry in Core Facilities

The Duke Human Vaccine Institute Research Flow Cytometry Facility serves the analytical and cell sorting needs of the DHVI and researchers throughout the Duke community. The DHVI Flow Facility offers state-of-the-art cytometric support to investigators in basic, developmental, and clinical research.

The Duke Human Vaccine Institute Flow Cytometry Facility instruments are capable of performing BSL-1 through BSL-3 live cell sorting, phenotypic acquisition, DNA cell cycle analysis, and intracellular marker analysis. Analytical and sorting capabilities up to 30 simultaneous parameters enable researchers to define subpopulations based on cell surface morphology as well as size and complexity.

We have three cell sorters (BD Symphony S6 (2) and a BD Aria II) that are operated by facility staff or by trained independent users. We have six cell analyzers (BD Symphony A5, BD LSRII, BD Fortessa, Luminex Muse (2) and an iQue Screener PLUS) available 24/7 for trained users. Instruments are located on the Duke West Campus and the Duke RTP Campus.

Contact

Facility

Staff

Our Locations on the Duke West Campus

MSRBII

RP1

GHRB

Our Locations on the Duke RTP Campus

RTP