Immunology at the RBL

The RBL Immunology Unit is a service component of the Regional Biocontainment Laboratory at Duke (housed in GHRB). In addition to providing access to specialized instrumentation for Real-Time PCR, and Luminex-based multiplex assays, this unit assists investigators with design and implementation of molecular, protein, and cellular assays to quantify immune reconstitution or immune responses in vitro and in vivo. 
Unit Manager:  

Barbara Theriot, MS

919-681-9099

Commercial Luminex assays (Magnetic or Non-magnetic)

  • Full service from sample to data (plasma, serum, cell supernatant, tissue homogenate, cerebrospinal fluid, lavage fluid…)

  • Assay read only (BioPlex 200, BioPlex FlexMap 3D, MagPix)

  • BSL2 and BSL3, Select Agents

Sample preparation

  • Plasma, serum, or PBMC prep from whole blood
  • Tissue specimen prep/homogenization
  • Nucleic acid extraction
  • Bead-based cell separation

ELISA

  • Commercial kit
  • Custom antigen-specific endpoint ELISAs

Real-time Thermocycler use

Quantitative signal joint T Cell Receptor Excision Circle (sjTREC) analysis (mouse, human, NHP)

Influenza Assay / Animal Model Request (form link)

  • To be used by CIVICS investigators to submit requests for animal studies and RUO assay support
  • Download required RUO assay sample submission manifest 

Please Note: These links/forms are for the exclusive use of Duke CIVICS program investigators. 

Questions?
 
(read more about CIVICS)

Use Fees/Rates

Current approved rates for Duke Investigators can be seen in CoreResearch@Duke (Core D-0002)

CoreResearch@Duke can also be used to request an ESTIMATE for grant or contract proposals

Service Requests and Estimates

Internal Duke users:  

External Non-Profit Institution users:

  • A signed Research Analysis Quote and Agreement (RAQA) and Purchase Order (PO) will be needed from your institution
  • Contact the RBL Immunology Unit for a price quote to get the process started.

Non-Duke For-Profit Entity users:

  • Duke Shared Resources can only provide services to For-Profit Entities under a signed Sponsored Research Agreement (SRA)
  • The collaborative research project must have a defined research question, scope of work, budget, and timeline.
  • Contact the RBL Immunology Unit to get the process started

Luminex Assay Kit Finder Tool

Directions to the RBL and Parking Information (contact us)

How to acknowledge work done in our facility:

Please acknowledge our facility in any publications/presentations:  “_____ was performed under the management of Barbara Theriot and direction of Dr. Andrew N. Macintyre in the Immunology Unit of the Duke Regional Biocontainment Laboratory (RBL), which received partial support for construction from the National Institutes of Health, National Institute of Allergy and Infectious Diseases (UC6-AI058607; G20-AI167200).”