Microbiology at the RBL

The RBL Microbiology Unit is a collaborative research service component of the Regional Biocontainment Laboratory at Duke (housed in GHRB). The Unit maintains quality-controlled secure inventories of approved BSL2, BSL3, and Select Agent bacterial pathogens for RBL projects and collaborating investigators. The Microbiology Unit has expertise in Chlamydia, and TB research program and helps the collaborators with study design, protocol optimization in-vitro and in-vivo. The core provides services such as IVIS live animal imaging in ABSL2/3, aerosol-delivered infection, macrophage-mycobacterium infection, extraction of mycobacteria from human samples, qRT-PCR, transcervical chlamydia infection (mice), and Inclusion Forming Unit (IFU) assay of chlamydia.

Unit Contact 

Manager: Uma Shankar Gautam, PhD

 

Build to Suit  

RBL Microbiology focuses on minimum inhibitory concentration screening, colony forming unit assays, and bacterial and fungal challenge models in mice. We provide customized full or partial support to meet your microbiology lab and animal model needs, including aerobiology (see Animal Support). 

Contact RBL Microbiology Unit Manager with needs.  

Sample Testing/Submission

Step 1:  Download and complete the appropriate sample submission manifest

Step 2:  Complete the Assay Request Form 


Use Fees/Rates

Current approved rates for Duke Investigators can be seen in CoreResearch@Duke (Core D-0045)

CoreResearch@Duke can also be used to request an ESTIMATE for a grant or contract​ proposal

Service Requests and Estimates

Internal Duke users:

 External Non-Profit Institution users:

  • A signed Research Analysis Quote and Agreement (RAQA) and Purchase Order (PO) will be needed from your institution
  • Contact Unit manager for a price quote to get the process started

Non-Duke For-Profit Entity users:

  • Duke Shared Resources can only provide services to For-Profit Entities under a signed Sponsored Research Agreement (SRA)
  • The collaborative research project must have a defined research question, scope of work, budget, and timeline.
  • Contact Unit manager to get the process started.

Directions to the RBL and Parking Information (contact us)

How to acknowledge work done in our facility:

Please acknowledge our facility in any publications/presentations:  “____work was performed in the Microbiology Unit of the Regional Biocontainment Laboratory at Duke, which received partial support for construction from the National Institutes of Health, National Institute of Allergy and Infectious Diseases (UC6-AI058607).”