Flow Cytometry in Core Facilities

The Duke Human Vaccine Institute Research Flow Cytometry Facility serves the analytical and cell sorting needs of the DHVI and researchers throughout the Duke Community. DHVI Flow offers state-of-the-art cytometric support to investigators in basic, developmental, and clinical research.

The Duke Human Vaccine Institute Flow Cytometry Facility instruments are capable of performing BSL-1 through BSL-3 live cell sorting, phenotypic acquisition, DNA cell cycle analysis, and intracellular marker analysis. Analytical and sorting capabilities up to 30 simultaneous parameters enable researchers to define subpopulations based on cell surface morphology as well as size and complexity.

We have three cell sorters (BD Symphony S6 and BD FACS Aria (2)) that are operated by facility staff or by trained independent users. We have six cell analyzers (BD Symphony A5, BD LSRII, BD Fortessa, Luminex Muse (2) and iQue Screener PLUS) available 24/7 for trained users. Instruments are located on the Duke West Campus and the Duke RTP Campus.

Download a flow cytometry poster here.

Open Flow: 

Intro to Cell Sorting Part I: https://www.youtube.com/watch?v=P6e9EbNdS64

Intro to Cell Sorting Part II: https://youtu.be/Y39MXA0Bmz4

Intro to Cell Sorting Part III: https://youtu.be/_Iy27WXf5Zk

Open Flow: Flourescence Compensation in Diva, reviews the principles behind compensation and how it is carried out on a BD Fortessa analyzer, using a live-run with a four color experiment.  A recording of this session can be found on their YouTube.) 

Open Flow: Multicolor Troubleshooting in Diva, focuses on rules of compensation and how our data can suffer when we do not follow them. A recording of this fourth session can be found on their YouTube

Contact Facility:

  • Main Campus: Email or 919-684-4130
  • RTP Campus: Email or 919-385-5712