CFAR Immunology Core - Tomaras

The Center for AIDS Research supports a multidisciplinary environment that promotes basic, clinical, epidemiologic, behavioral, and translational research in the prevention, detection, and treatment of HIV infection and AIDS. The Duke Immunology Core provides innovative, state-of-the-art, and standardized repertoires of immunologic assays to comprehensively interrogate the immunologic space in HIV-1 infection, vaccination, co-infection and HIV-1 cure studies. The Core’s broad range of assay services include the following: HIV and SIV neutralization and antiviral assays; neutralization epitope mapping; HIV/SIV binding antibody multiplex assays (BAMA); comprehensive fluorescence-activated cell sorting (FACS) including sorting of live, HIV-infected cells; analytic polychromatic flow cytometry (PFC) phenotyping; single cell functional analyses of cellular reactivities using intracellular cytokine staining (ICS); flow-based cytotoxicity assays; and single cell B and T cell ELISpot assays. In support of each of these platforms, consultation and training services are offered and quality assurance oversight is provided.