Kwan-Ki Hwang

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Senior Research Scientist

Duke Human Vaccine Institute

Research Interests:
 
Kwan-Ki Hwang, PhD is a Senior Research Scientist at the Duke Human Vaccine Institute. He obtained his PhD in Physiology at Michigan State University in 1999. Upon graduation, he joined the laboratory of Dr. Pojen P. Chen at the University of California Los Angeles. As a postdoctoral fellow and an assistant research professor, he studied pathogenic mechanisms of autoimmune diseases including anti-phospholipid syndrome.
 
Dr. Hwang joined the Duke Human Vaccine Institute in September 2007 and established the Human Monoclonal Core Facility for the generation of native human monoclonal antibodies (mAbs). His current research focuses on generation of panels of human mAbs derived from patients with acute and chronic HIV-1 infection, as well as from recipients of experimental HIV-1 vaccines, which neutralize and inhibit HIV-1 infectivity. This research will lead to identification of molecular targets recognized by broadly neutralizing and inhibiting antibodies and development of vaccines inducing an effective, protective immune response in the patients.
 
Representative Publications:
 
Hwang KK, Chen X, Kozink DM, Gustilo M, Marshall DJ, Whitesides JF, Liao HX, Catera R, Chu CC, Yan XJ, Luftig MA, Haynes BF, Chiorazzi N. Enhanced outgrowth of EBV-transformed chronic lymphocytic leukemia B cells mediated by co-culture with macrophage feeder cells. Blood. 2011 Dec 12. [Epub ahead of print] Abstract
 
Liao HX, Chen X, Munshaw S, Zhang R, Marshall DJ, Vandergrift N, Whitesides JF, Lu X, Yu JS, Hwang KK, Gao F, Markowitz M, Heath SL, Bar KJ, Goepfert PA, Montefiori DC, Shaw GC, Alam SM, Margolis DM, Denny TN, Boyd SD, Marshal E, Egholm M, Simen BB, Hanczaruk B, Fire AZ, Voss G, Kelsoe G, Tomaras GD, Moody MA, Kepler TB, Haynes BF. Initial antibodies binding to HIV-1 gp41 in acutely infected subjects are polyreactive and highly mutated. J Exp Med. 2011 Oct 24;208(11): 2237-49. Abstract
 
Verkoczy L, Chen Y, Bouton-Verville H, Zhang J, Diaz M, Hutchinson J, Ouyang YB, Alam SM, Holl TM, Hwang KK, Kelsoe G, Haynes BF. Rescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls. J Immunol. 2011 Oct 1;187(7): 3785-97. Abstract
 
Bonsignori M, Hwang KK, Chen X, Tsao CY, Morris L, Gray E, Marshall DJ, Crump JA, Kapiga SH, Sam NE, Sinangil F, Pancera M, Yongping Y, Zhang B, Zhu J, Kwong PD, O'Dell S, Mascola JR, Wu L, Nabel GJ, Phogat S, Seaman MS, Whitesides JF, Moody MA, Kelsoe G, Yang X, Sodroski J, Shaw GM, Montefiori DC, Kepler TB, Tomaras GD, Alam SM, Liao HX, Haynes BF. Analysis of a clonal lineage of HIV-1 envelope V2/V3 conformational epitope-specific broadly neutralizing antibodies and their inferred unmutated common ancestors. J Virol. 2011 Oct;85(19):9998-10009. Abstract
 
Moody MA, Liao HX, Alam SM, Scearce RM, Plonk MK, Kozink DM, Drinker MS, Zhang R, Xia SM, Sutherland LL, Tomaras GD, Giles IP, Kappes JC, Ochsenbauer-Jambor C, Edmonds TG, Soares M, Barbero G, Forthal DN, Landucci G, Chang C, King SW, Kavlie A, Denny TN, Hwang KK, Chen PP, Thorpe PE, Montefiori DC, Haynes BF. Anti-phospholipid human monoclonal antibodies inhibit CCR5-tropic HIV-1 and induce beta-chemokines. J Exp Med. 2010 Apr 12;207(4):763-76. Abstract
 
Yang Y-H, Chien D, FitzGerald J, Grossman JM, Hahn BH, Hwang K-K, Chen PP.  Novel autoantibodies against the activated coagulation factor IX (FIXa) in the antiphospholipid syndrome that Interpose the FIXa regulation by Antithrombin. J Immunol 2009. 182 (3): 1674-1680. Abstract
 
Liao H-X, Levesque MC, Nagel A, Dixon A, Zhang R, Walter E, Parks R, Whitesides J, Marshall DJ, Hwang K-K, Yang Y, Chen X, Gao F, Munshaw S, Kepler TB, Denny T, Moody MA, Haynes BF. High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies. J Virol Methods 2009. 158 (1-2): 171-179. Abstract
 
Levesque MC, Moody MA, Hwang K-K, Marshall DJ, Whitesides JF, Gurley TC, Allgood S, Haynes BB, Vandergrift NA, Plonk S, Parker DC, Cohen MS, Tomaras GD, Goepfert PA, Shaw GM, Schmitz JE, Eron JJ, Shaheen NJ, Hicks CB, Liao H-X, Markowitz M, Kelsoe G, Margolis DM, Haynes BF. Polyclonal B cell differentiation and loss of gastrointestinal tract germinal centers in the earliest stages of HIV-1 infection. PloS Medicine 2009. Abstract
 
Ede K, Hwang K-K, Wu C-C, Wu M, Yang Y-H, Lin W-S, Chien D, Chen P-C, Tsao BP, McCurdy DK, Chen PP. Plasmin immunization induces likely prothrombotic IgG anti-cardiolipin antibodies preferentially in MRL/MpJ mice. Arthritis Rheum 2009. 60: 3108-3117. Abstract
 
Lin WS, Chen PC, Yang CD, Cho E, Hahn BH, Grossman J, Hwang KK, Chen PP. Some antiphospholipid antibodies recognize conformational epitopes shared by beta2-glycoprotein I and the homologous catalytic domains of several serine proteases. Arthritis Rheum. 2007 May. 56(5):1638-47. Abstract
 
Chen X-X, Gu Y-Y, Li S-J, Qian J, Hwang K-K, Chen PP, Chen S-L, Yang C-D. Some plasmin-induced antibodies bind to cardiolipin, display lupus anticoagulant activity and induce fetal loss in mice. J Immunol 2007. 178: 5351-5356. Abstract
 
Lin W-S, Chen P-C, Yang C-D, Cho EJ, Hahn BH, Grossman JM, Hwang K-K, Chen PP. Some antiphospholipid antibodies recognize conformational epitopes shared by β2GPI and the homologous catalytic domains of several serine proteases. Arthritis Rheum 2007. 56 (5): 1638-1647. Abstract
 
Yang YH, Hwang KK, FitzGerald J, Grossman JM, Taylor M, Hahn BH, Chen PP. Antibodies against the activated coagulation factor X (FXa) in the antiphospholipid syndrome that interfere with the FXa inactivation by antithrombin. J Immunol. 2006 Dec 1. 177(11):8219-25. Abstract
 
Vega-Ostertag ME, Liu X, Hwang K-K, Chen PP, Pierangeli SS. A human monoclonal antiprothrombin antibody is thrombogenic in vivo and upregulates expression of tissue factor and E-selectin on endothelial cells. Br J Haematol 2006. 135 (2): 214-219.
 
Lu CS, Horizon AA, Hwang KK, FitzGerald J, Lin WS, Hahn BH, Wallace DJ, Metzger AL, Weisman MH, Chen PP. Identification of polyclonal and monoclonal antibodies against tissue plasminogen activator in the antiphospholipid syndrome. Arthritis Rheum. 2005 Dec. 52(12):4018-27. Abstract