Laurent Karl Verkoczy, PhD

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Assistant Professor in Medicine
Assistant Professor of Pathology
Department / Division:
Medicine / Medicine - DHVI Faculty
Address:
106 Research Drive
Msrbii Building, Room 3015
Durham, NC 27710
Office Telephone:
(919) 681-3134
Training:
  • PhD, Univ Toronto, 2000
Research Interests:
Laurent Verkoczy, PhD is Assistant Professor of Medicine at Duke University Medical Center and directs the Laboratory of B cell Immunoregulation at Duke Human Vaccine Institute (DHVI). Verkoczy joined the DHVI in August 2005 after obtaining a Ph.D. in Immunology from the University of Toronto and completing a post-doctoral fellowship in the laboratory of David Nemazee at The Scripps Research Institute. The overall focus of the laboratory of B cell immunoregulation is to identify viral and host determinants involved in controlling B cell responses required for generating “broadly neutralizing” antibodies (bnAbs) to the HIV-1 gp41 Membrane Proximal External Region (MPER).
Representative Publications:
  • Bonsignori, M; Wiehe, K; Grimm, SK; Lynch, R; Yang, G; Kozink, DM; Perrin, F; Cooper, AJ; Hwang, KK; Chen, X; Liu, M; McKee, K; Parks, RJ; Eudailey, J; Wang, M; Clowse, M; Criscione-Schreiber, LG; Moody, MA; Ackerman, ME; Boyd, SD; Gao, F; Kelsoe, G; Verkoczy, L; Tomaras, GD; Liao, HX; Kepler, TB; Montefiori, DC; Mascola, JR; Haynes, BF. An autoreactive antibody from an SLE/HIV-1 individual broadly neutralizes HIV-1. Journal of Clinical Investigation. 2014;124:1835-1843.  Abstract
  • Haynes, BF; Verkoczy, L. AIDS/HIV. Host controls of HIV neutralizing antibodies. Science. 2014;344:588-589.  Abstract
  • Haynes, BF; Verkoczy, L. Host controls of HIV neutralizing antibodies. Science. 2014;344:588-589.  
  • Holl, TM; Yang, G; Kuraoka, M; Verkoczy, L; Alam, SM; Moody, MA; Haynes, BF; Kelsoe, G. Enhanced antibody responses to an HIV-1 membrane-proximal external region antigen in mice reconstituted with cultured lymphocytes. Journal of Immunology. 2014;192:3269-3279.  Abstract
  • Verkoczy, L; Diaz, M. Autoreactivity in HIV-1 broadly neutralizing antibodies: implications for their function and induction by vaccination. Current Opinion in HIV and AIDS. 2014;9:224-234.  Abstract
  • Verkoczy, L; Kelsoe, G; Haynes, BF. HIV-1 envelope gp41 broadly neutralizing antibodies: hurdles for vaccine development. PLoS Pathogens. 2014;10:e1004073.  Abstract
  • Zhang, J; Alam, SM; Bouton-Verville, H; Chen, Y; Newman, A; Stewart, S; Jaeger, FH; Montefiori, DC; Dennison, SM; Haynes, BF; Verkoczy, L. Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies. Journal of Immunology. 2014;192:1693-1706.  
  • Zhang, J; Alam, SM; Bouton-Verville, H; Chen, Y; Newman, A; Stewart, S; Jaeger, FH; Montefiori, DC; Dennison, SM; Haynes, BF; Verkoczy, L. Modulation of nonneutralizing HIV-1 gp41 responses by an MHC-restricted TH epitope overlapping those of membrane proximal external region broadly neutralizing antibodies. Journal of Immunology. 2014;192:1693-1706.  Abstract
  • Chen, Y; Zhang, J; Bouton-Verville, H; Newman, A; Xia, S; Liao, H; Montefiori, DC; Moody, MA; Armand, L; Hwang, K; Lockwood, B; Dennison, SM; Alam, SM; Haynes, BF; Verkoczy, L. Lipid Components in MPER-Based Immunization Regimens Are Critical for Inducing Broadly Neutralizing Antibody Responses in 2F5 and 4E10 Knockin Mice. AIDS Research and Human Retroviruses. A20-A20.  
  • Chen, Y; Zhang, J; Hwang, KK; Bouton-Verville, H; Xia, SM; Newman, A; Ouyang, YB; Haynes, BF; Verkoczy, L. Common tolerance mechanisms, but distinct cross-reactivities associated with gp41 and lipids, limit production of HIV-1 broad neutralizing antibodies 2F5 and 4E10. Journal of Immunology. 2013;191:1260-1275.  Abstract
  • Haynes, BF; Kepler, TB; Alam, M; Harrison, S; Bonsignori, M; Moody, MA; Kelsoe, G; Verkoczy, L; Liao, H-X. Analysis of Broad Neutralizing B Cell Lineages to Guide HIV-1 Immunogen Design. JAIDS: Journal of Acquired Immune Deficiency Syndromes. 54-54.  
  • Verkoczy, L; Chen, Y; Zhang, J; Bouton-Verville, H; Newman, A; Lockwood, B; Scearce, RM; Montefiori, DC; Dennison, SM; Xia, SM; Hwang, KK; Liao, HX; Alam, SM; Haynes, BF. Induction of HIV-1 broad neutralizing antibodies in 2F5 knock-in mice: selection against membrane proximal external region-associated autoreactivity limits T-dependent responses. Journal of Immunology. 2013;191:2538-2550.  Abstract
  • Yang, G; Holl, T; Nojima, T; Verkoczy, L; Moody, M; Haynes, B; Kitamura, D; Kelsoe, G. The role of immunological tolerance in humoral responses to the 2F5 epitope of HIV-1. Journal of Immunology.  
  • Bonsignori, M; Alam, SM; Liao, HX; Verkoczy, L; Tomaras, GD; Haynes, BF; Moody, MA. HIV-1 antibodies from infection and vaccination: insights for guiding vaccine design. Trends in Microbiology. 2012;20:532-539.  Abstract
  • Alam, SM; Liao, HX; Dennison, SM; Jaeger, F; Parks, R; Anasti, K; Foulger, A; Donathan, M; Lucas, J; Verkoczy, L; Nicely, N; Tomaras, GD; Kelsoe, G; Chen, B; Kepler, TB; Haynes, BF. Differential reactivity of germ line allelic variants of a broadly neutralizing HIV-1 antibody to a gp41 fusion intermediate conformation. Journal of Virology. 2011;85:11725-11731.  Abstract
  • Haynes, BF; Moody, MA; Liao, HX; Verkoczy, L; Tomaras, GD. B cell responses to HIV-1 infection and vaccination: pathways to preventing infection. Trends in Molecular Medicine. 2011;17:108-116.  Abstract
  • Verkoczy, L; Chen, Y; Bouton-Verville, H; Zhang, J; Diaz, M; Hutchinson, J; Ouyang, YB; Alam, SM; Holl, TM; Hwang, KK; Kelsoe, G; Haynes, BF. Rescue of HIV-1 broad neutralizing antibody-expressing B cells in 2F5 VH x VL knockin mice reveals multiple tolerance controls. Journal of Immunology. 2011;187:3785-3797.  Abstract
  • Verkoczy, L; Kelsoe, G; Moody, MA; Haynes, BF. Role of immune mechanisms in induction of HIV-1 broadly neutralizing antibodies. Current Opinion in Immunology. 2011;23:383-390.  Abstract
  • Shen, X; Dennison, SM; Liu, P; Gao, F; Jaeger, F; Montefiori, DC; Verkoczy, L; Haynes, BF; Alam, SM; Tomaras, GD. Prolonged exposure of the HIV-1 gp41 membrane proximal region with L669S substitution. Proceedings of the National Academy of Sciences of USA. 2010;107:5972-5977.  Abstract
  • Verkoczy, L; Bouton-Verville, H; Hutchinson, J; Scearce, RM; Liao, H; Hwang, K; Haynes, BF. Role of immunoglobulin light chain usage and MPER specificity in counterselecting B cells expressing the broadly neutralizing antibody 2F5. AIDS Research and Human Retroviruses. A154-A154.  
  • Verkoczy, L; Diaz, M; Holl, TM; Ouyang, YB; Bouton-Verville, H; Alam, SM; Liao, HX; Kelsoe, G; Haynes, BF. Autoreactivity in an HIV-1 broadly reactive neutralizing antibody variable region heavy chain induces immunologic tolerance. Proceedings of the National Academy of Sciences of USA. 2010;107:181-186.  Abstract
  • Holl, TM; Kuraoka, M; Liao, D; Verkoczy, L; Moody, MA; Alam, M; Liao, H; Haynes, BF; Kelsoe, GH. Generation of antibody responses to HIV-1 membrane proximal external region (MPER) antigen. Retrovirology. 2009;6.  
  • Holl, TM; Kuraoka, M; Liao, D; Verkoczy, L; Moody, MA; Alam, M; Liao, H; Haynes, BF; Kelsoe, GH. P04-44. Generation of antibody responses to HIV-1 membrane proximal external region (MPER) antigen. Retrovirology. 2009;6:P72.  
  • Richter, K; Brar, S; Ray, M; Pisitkun, P; Bolland, S; Verkoczy, L; Diaz, M. Speckled-like pattern in the germinal center (SLIP-GC), a nuclear GTPase expressed in activation-induced deaminase-expressing lymphomas and germinal center B cells. Journal of Biological Chemistry. 2009;284:30652-30661.  Abstract
  • Schroeder, H; Kapoor, P; Shaw, G; Parks, R; Alam, S; Verkoczy, L; Haynes, B. T.59. The Composition of the Primary Immunoglobulin Repertoire Influences HIV gp140 Epitope Selection. Clinical Immunology. 2009;131:S66-S67.  
  • Shen, X; Dennison, M; Gao, F; Montefiori, DC; Verkoczy, L; Haynes, B; Alam, M; Tomaras, G. HIV-1 gp41 envelope MPER mutation altered epitope conformation in lipid and increased sensitivity to 2F5 and 4E10 neutralizing antibodies. Retrovirology. 2009;6.  
  • Shen, X; Dennison, M; Gao, F; Montefiori, DC; Verkoczy, L; Haynes, B; Alam, M; Tomaras, G. OA021-04. HIV-1 gp41 envelope MPER mutation altered epitope conformation in lipid and increased sensitivity to 2F5 and 4E10 neutralizing antibodies. Retrovirology. 2009;6:O16.  
  • Verkoczy, L; Diaz, M; Holl, TM; Ouyang, Y; Bouton-Verville, H; Alam, SM; Liao, H; Kelsoe, G; Haynes, BF. Immunological tolerance prevents the expression of a broadly reactive neutralizing HIV-1 antibody. Retrovirology. 2009;6.  
  • Verkoczy, L; Diaz, M; Holl, TM; Ouyang, Y; Bouton-Verville, H; Alam, SM; Liao, H; Kelsoe, G; Haynes, BF. P04-26. Immunological tolerance prevents the expression of a broadly reactive neutralizing HIV-1 antibody. Retrovirology. 2009;6:P54.  
  • Verkoczy, L; Moody, MA; Holl, TM; Bouton-Verville, H; Scearce, RM; Hutchinson, J; Alam, SM; Kelsoe, G; Haynes, BF. Functional, non-clonal IgMa-restricted B cell receptor interactions with the HIV-1 envelope gp41 membrane proximal external region. PLoS One. 2009;4:e7215.  Abstract
  • Holl, MT; Kuraoka, M; Liao, D; Verkoczy, L; Moody, MA; Alam, SM; Haynes, B; Kelsoe, G. Generation of Robust Antibody Responses to HIV-1 Membrane Proximal External Region (MPER) Antigens. AIDS Research and Human Retroviruses. 24-25.  
  • Haynes, BF; Moody, MA; Verkoczy, L; Kelsoe, G; Alam, SM. Antibody polyspecificity and neutralization of HIV-1: a hypothesis. Human Antibodies. 2005;14:59-67.  Abstract