Marcella Sarzotti-Kelsoe, PhD

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Research Professor of Immunology
Associate Professor in Surgery
Department:
Immunology
Address:
2812 Erwin Rd.
Suite 301
Durham, NC 27705
Office Telephone:
(919) 684-6373
Training:
  • PhD, University of Italy, 1980
Research Interests:
Basic Research (past and present)
Development of T cell responses in neonates.
Neonatal T cell receptor Vβ repertoire diversity in the peripheral T cell pool.
The role of heat shock protein, as a natural adjuvant, at eliciting innate and adaptive immune responses.
Development of the T cell receptor repertoire in naïve, immunodeficient infants, given bone marrow or thymic transplantation.
Thymic output, T cell diversity and T cell function in long-term human SCID chimeras.
Telomere length in T cells from SCID chimeras.

Applied Research (past and present)
I direct a Global Quality Assurance Program, which I developed and pioneered here at Duke University, to oversee compliance with Good Clinical Laboratory Practice Guidelines in three HIV vaccine trial networks (CHAVI, CAVD, Duke HVTN, EQAPOL) involving domestic and international sites in Asia, Africa, Europe, and South America.
I also direct a Global Proficiency Testing Program for laboratories testing for neutralizing antibody function in individuals infected with HIV or vaccinated against HIV. The Program was recently launched (Aug. 2009).
Representative Publications:
  • Sarzotti-Kelsoe, M; Daniell, XG; Whitesides, JF; Buckley, RH. The long and the short of telomeres in bone marrow recipient SCID patients. Immunologic Research: a selective reference to current research and practice. 2011;49:44-48.  Abstract
  • Sarzotti-Kelsoe, M; Cox, J; Cleland, N; Denny, T; Hural, J; Needham, L; Ozaki, D; Rodriguez-Chavez, IR; Stevens, G; Stiles, T; Tarragona-Fiol, T; Simkins, A. Evaluation and recommendations on good clinical laboratory practice guidelines for phase I-III clinical trials. PLoS Medicine. 2009;6:e1000067.  Abstract
  • Sarzotti-Kelsoe, M; Win, CM; Parrott, RE; Cooney, M; Moser, BK; Roberts, JL; Sempowski, GD; Buckley, RH. Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras. Blood. 2009;114:1445-1453.  Abstract
  • Markert, ML; Devlin, BH; Alexieff, MJ; Li, J; McCarthy, EA; Gupton, SE; Chinn, IK; Hale, LP; Kepler, TB; He, M; Sarzotti, M; Skinner, MA; Rice, HE; Hoehner, JC. Review of 54 patients with complete DiGeorge anomaly enrolled in protocols for thymus transplantation: outcome of 44 consecutive transplants. Blood. 2007;109:4539-4547.  Abstract
  • Roberts, JL; Lauritsen, JP; Cooney, M; Parrott, RE; Sajaroff, EO; Win, CM; Keller, MD; Carpenter, JH; Carabana, J; Krangel, MS; Sarzotti, M; Zhong, XP; Wiest, DL; Buckley, RH. T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3zeta subunit of the T-cell antigen receptor complex. Blood. 2007;109:3198-3206.  Abstract
  • Fadel, SA; Cowell, LG; Cao, S; Ozaki, DA; Kepler, TB; Steeber, DA; Sarzotti, M. Neonate-primed CD8+ memory cells rival adult-primed memory cells in antigen-driven expansion and anti-viral protection. International Immunology. 2006;18:249-257.  Abstract
  • Mascola, JR; D'Souza, P; Gilbert, P; Hahn, BH; Haigwood, NL; Morris, L; Petropoulos, CJ; Polonis, VR; Sarzotti, M; Montefiori, DC. Recommendations for the design and use of standard virus panels to assess neutralizing antibody responses elicited by candidate human immunodeficiency virus type 1 vaccines. Journal of Virology. 2005;79:10103-10107.  Abstract
  • Baker-LePain, JC; Sarzotti, M; Nicchitta, CV. Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo. Journal of Immunology. 2004;172:4195-4203.  Abstract
  • Markert, ML; Alexieff, MJ; Li, J; Sarzotti, M; Ozaki, DA; Devlin, BH; Sedlak, DA; Sempowski, GD; Hale, LP; Rice, HE; Mahaffey, SM; Skinner, MA. Postnatal thymus transplantation with immunosuppression as treatment for DiGeorge syndrome. Blood. 2004;104:2574-2581.  Abstract
  • Markert, ML; Sarzotti, M; Ozaki, DA; Sempowski, GD; Rhein, ME; Hale, LP; Le Deist, F; Alexieff, MJ; Li, J; Hauser, ER; Haynes, BF; Rice, HE; Skinner, MA; Mahaffey, SM; Jaggers, J; Stein, LD; Mill, MR. Thymus transplantation in complete DiGeorge syndrome: immunologic and safety evaluations in 12 patients. Blood. 2003;102:1121-1130.  Abstract
  • Sarzotti, M; Patel, DD; Li, X; Ozaki, DA; Cao, S; Langdon, S; Parrott, RE; Coyne, K; Buckley, RH. T cell repertoire development in humans with SCID after nonablative allogeneic marrow transplantation. Journal of Immunology. 2003;170:2711-2718.  Abstract
  • Baker-LePain, JC; Sarzotti, M; Fields, TA; Li, CY; Nicchitta, CV. GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression. Journal of Experimental Medicine. 2002;196:1447-1459.  Abstract
  • Fadel, SA; Ozaki, DA; Sarzotti, M. Enhanced type 1 immunity after secondary viral challenge in mice primed as neonates. Journal of Immunology. 2002;169:3293-3300.  Abstract
  • Cerasoli, DM; Kelsoe, G; Sarzotti, M. CD4+Thy1- thymocytes with a Th-type 2 cytokine response. International Immunology. 2001;13:75-83.  Abstract
  • Fadel, S; Sarzotti, M. Cellular immune responses in neonates. International Reviews of Immunology. 2000;19:173-193.  Abstract
  • Sarzotti, M. Immunologic tolerance. Current Opinion in Hematology. 1997;4:48-52.  Abstract
  • Sarzotti, M; Dean, TA; Remington, MP; Ly, CD; Furth, PA; Robbins, DS. Induction of cytotoxic T cell responses in newborn mice by DNA immunization. Vaccine. 1997;15:795-797.  Abstract
  • Sarzotti, M; Robbins, DS; Hoffman, PM. Induction of protective CTL responses in newborn mice by a murine retrovirus. Science. 1996;271:1726-1728.  Abstract