RBL Core Resources Flyers
BSL3 Animal Models Core Resource
The Animal Models Core Resource will provide challenge models using multiple pathogens, animal species, and routes of infection. Animal models are established under detailed SOPs including inoculum confirmation and sentinel animal necropsy to confirm dose delivered. This Core Resource can manage multiple species including mice, rats, hamsters, gerbils, ferrets, guinea pigs and rabbits.
BSL3 Aerobiology Core Resource
The Aerobiology Core Resource allows for replication of real-world exposure conditions to harmful pathogens. Injection or intranasal exposure of animals to infectious agents may not lead to disease progression which mimics aerosol acquired infections. Experimental infection by inhalation is a critical requirement for development and testing of countermeasures to biological threats. Aerobiology protocols include inoculum confirmation, real-time recording of exposure parameters, characterization of particle distribution, aerosol sampling to estimate inhaled dose, and sentinel necropsy to define dose delivered.
BSL3 Flow Cytometry Core Resource
The Flow Cytometry Core Resource, led by John Whitesides, includes both BSL2 and BSL3 components. The Flow Cytometry Core Resource offers several cytometric services including Phenotypic Analysis, FRET (Fluorescent Resonance Energy Transfer), DNA cell cycle Analysis and Intracytoplasmic Cytokine Analysis. The analytical and sorting abilities exceed eight simultaneous parameters and enable researchers to define subpopulations based on cell surface morphology as well as size and complexity. For BSL-3 we currently operate a BD FACSAria™ cell sorter to analyze and separate infectious samples.
BSL3 Biocontainment Animal Imaging Core Resource
The Biocontainment Animal Imaging Core Resource is led by Gregory Sempowski, PhD. This facility is unique in that the imaging services are contained within a BSL-2/BSL-3 facility. The use of in vivo imaging techniques to follow the course of infection, replication, persistence, and dissemination of pathogens in mice has provided a valuable tool to analyze the interaction between these agents and the host. Live whole animal imaging significantly reduces the numbers of animals required for kinetic studies and allows, at terminal necropsy, for localization of specific sites of pathogen colonization.
Immune Monitoring Core Resource
The DHVI Immune Monitoring Core Resource, led by Greg Sempowski, provides access to specialized instrumentation for Real-Time PCR, Luminex-based multiplex ELISA, and T Cell Receptor Spectratyping. This Core Resource assists investigators with the design and implementation of molecular, protein, and cellular assays to quantify immune reconstitution or immune responses in vitro and in vivo. A major accomplishment of this facility was the development of a patented Real-Time PCR assay system to quantify mouse T-cell receptor excision circles. This assay has proven invaluable for monitoring thymopoiesis in mice.
Select Agent Core Resource
The Select Agent Core Resource allows investigators to work with Select Agents under the Duke Select Agent Registration. The RBL Director serves as the Principal Investigator for the registration and supervises all work with Select Agents. This Core Resource completes documentation required for receipt and inventory maintenance under the Select Agent Final Rule, including regular audits of laboratory work in progress to ensure compliance. Central freezer storage is available for long term storage of Select Agent samples.